Figure 4

Cytochrome C levels within cytosolic and mitochondrial fractions of cells after 4 hours of treatment. (A) Western blotting of cytochrome C. Cells were treated with hydrogen peroxide (10 μM) and untreated control. (B) Lysates were measured by ELISA. Cells were untreated (control) or treated with DMSO (1%), Taxol (1 μM), tobacco smoke condensate (80 μg/ml), paraldehyde (10 μM), nicotine (1 mM) and hydrogen peroxide (10 μM). (C) Lysates were measured by ELISA. Cells were untreated (control) or treated with CSE (50 and 100%). Closed bars show mitochondrial fraction and open show cytoplasmic fraction. Bars are the mean ± s.e. mean cytochrome C/ml assay media from at least 3 separate experiments. Asterisks denote statistical significance from control cells of same cell fraction. **, p < 0.01 and ***; $$$, p < 0.001.