Figure 2
Graph of cell distribution in detached A549 cells after 48 hours of treatment measured by morphological analysis by DAPI staining. Data shown the number of normal, apoptotic or indeterminate cells among the detached cell population as measured by morphological assessment. Cells were seeded at a density of 105 cells per well, prior to treatment in serum free media. (A) Cells were untreated (control) or treated with DMSO (1%), Taxol (1 μM), TSC (80 μg/ml), paraldehyde (Para; 10 μM), nicotine (Nic; 1 mM) and hydrogen peroxide (HP; 10 μM). (B) Cells were untreated (control) or treated with 100%, 50% and 25% CSE. Vertically striped bars show "normal", open bars show "apoptotic" and hatched bars show "indeterminate" cells/ml of the culture and are the mean ± s.e. mean of at least 3 determinations. Asterisks denote statistical significance (p < 0.05) from control cells showing the same morphology.