Immunohistochemical staining of neutrophils in the liver tissue section. Mice were subjected to surgical procedure involving 90 min of ischemia followed by 6 hrs of reperfusion. Neutrophils are stained dark brown. Rows A and B correspond to the ischemic liver sections, while row C corresponds to the non-ischemic liver of the same animal subjected to hepatic I/R. Abbreviations: I = ischemic liver, NI = non-ischemic liver. Images are representative of four liver samples per each time point/group.