Liver histopathology following hepatic I/R. Mice were subjected to surgical procedure involving 90 min of ischemia followed by 6 hrs of reperfusion, and the liver sections were then prepared and stained with H&E. Images were obtained with a 10× and 40× objective lenses. Rows A and B correspond to the ischemic liver sections, while row C corresponds to the non-ischemic liver of the same animal subjected to hepatic I/R. There is a significant amount of ischemic damage, as is evident by the loss of hepatocytes in the pericentral and midzonal areas, with relative sparing of the periportal areas (Rows A and B). Neutrophils are present in the midzonal region around the central vein. The non-ischemic liver sections show normal histology (Row C). Images are representative of 6–9 liver samples per each time point/group. Abbreviations: I = ischemic liver, NI = non-ischemic liver.