Figure 2

Following bacterial inflammation, GFP⁺ cells in the lung highly expressed PRXV. Animals were transplanted with GFP⁺ bone marrow, and progeny of GFP⁺ cells (green fluorescence) was located to the sites of inflammation in the bronchial epithelium. Confocal microscopy images of the cryosectioned lung stained for PRXV with red fluorescent antibodies. A: GFP⁺ cells in the peribronchial interstitial spaces following inflammation of the lung, upper left panel – PRXV staining (red fluorescence), upper right panel – GFP fluorescence (green), lower panel – combined image. B: GFP⁺ cells in the wall of the large bronchus. Upper left panel – PRXV staining (red fluorescence), upper right panel – GFP fluorescence (green), lower left panel – reflected light image, lower right panel – combined image. C-D: Fluorescence intensity of PRXV label (red) co-localized with green GFP signal in the lung tissues. At the bottom of each image the profile diagram of distribution of fluorescence intensity along selected segment (blue bar) is given. Red line is PRXV fluorescence intensity, green line is GFP fluorescence intensity. Original magnification × 40. E: Summary results of relative PRXV fluorescence intensity in the bronchial epithelium (loose shade bar), alveolar walls (dense shade bar), and GFP⁺ cells (black bar) present in the lung from 3 different experiments. * – p < 0.05.