Effect of PACs on TNF-α-induced ICAM-1, IL-8, VEGF
mRNA expression in human endothelial LT2 cells. LT2 cells were pretreated with PACs (10, 30 and 60 μg/ml) or PBS for 24 h and then activated with 5 ng/ml TNF-α for 12 h. PACs dose-dependently inhibited mRNA expression of ICAM-1 (A), maintained IL-8 (B) and VEGF165 (D) and up-regulated dose-dependently VEGF121 mRNA expression (C). Gel electrophoresis of PCR products is shown under X-axis. Each value is the mean ± s.e. mean of three experiments analysed in duplicate each. °P < 0.01 versus negative control. *P < 0.05 versus positive control. **P < 0.01 versus positive control.