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Figure 5 | Journal of Inflammation

Figure 5

From: Colocalization of endogenous TNF with a functional intracellular splice form of human TNF receptor type 2

Figure 5

Biochemical characterization of hicp75TNFR.(A) The hp75TNFR from NIH3T3 cells either transduced with hp75TNFR or hicp75TNFR, respectively, were immunoprecipitated with monoclonal antibodies against the extracellular domain of hp75TNFR (80M2) and stained after blotting with antibodies to the intracellular domain on hp75TNFR (sc-7862). (B) Affinity purified soluble hicp75TNFR from supernatant of transduced TNFR1/2 knock-out fibroblasts was stained after blotting with a rabbit serum against the extracellular domain of hp75TNFR (80M).

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Journal of Inflammation

ISSN: 1476-9255

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