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Figure 4 | Journal of Inflammation

Figure 4

From: Colocalization of endogenous TNF with a functional intracellular splice form of human TNF receptor type 2

Figure 4

Shedded ectodomain of hp75TNFR isoforms. (A)The extracellular domain of both hp75TNFR isoforms is released constitutively (black bars) into the supernatant of L929 cell either transduced with hp75TNFR or hicp75TNFR, respectively. Shedding is increased by rhTNF (6 ng/ml; light gray bars) and attenuated by TAPI (100 nM, dark gray bars). (B)The extracellular domain of hicp75TNFR is bioactive as shown by neutralization of rhTNF in a TNF cytotoxicity assay on L929 cells. Supernatant from L929 cells either transduced with icp75TNFR-YFP (●), or YFP alone (○) were tested. DMEM (▼) served as control medium. All values are given as mean ± S.D. of triplicate cultures. Four independent experiments gave similar results.

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Journal of Inflammation

ISSN: 1476-9255

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