Localization of tagged hp75TNFR and hicp75TNFR molecules in transduced NIH 3T3 cells. Cells transduced either with myc-tagged (A;C) or YFP-tagged (B;D) hp75TNFR (A;B) or hicp75TNFR (C;D) were analyzed microscopically. For detection of YFP the cells were fixed and analyzed. For detection of the myc-tag cells were fixed and permeabilized followed by incubation with a monoclonal mouse anti-human c-myc (9E10) and a secondary anti-mouse FITC antibody. While hp75TNFR staining is mainly found on the plasma membrane, hicp75TNFR staining is perinuclear, punctuated, and distributed throughout the cytoplasm. Nuclei: Dapi (A;C), Hoechst 33342 (B;D).