Figure 5From: C-reactive protein does not opsonize early apoptotic human neutrophils, but binds only membrane-permeable late apoptotic cells and has no effect on their phagocytosis by macrophagesAdditive inhibition of CRP binding by EDTA and heparin. FITC-CRP was incubated with cultured human neutrophils in the presence of 2 mM Ca2+ or 5 mM EDTA, with or without pre-incubation with 1 mg/ml heparin. Binding to late apoptotic neutrophils was assessed by gating on the propidium iodide-positve cell population. The inhibitory effects of EDTA and heparin on CRP binding were additive.Back to article page