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Table 1 Effect of Dex on IL-9 mRNA in 13 different individuals.

From: Dexamethasone inhibits IL-9 production by human T cells

Expt

Ct no Dex

Ct with Dex

Amplification Efficiency

% IL-9 in Dex vs no Dex

1

22.6

34.3

1.74

0.15

2

21.1

32.1

1.76

0.20

3

24.0

35.0

1.91

0.08

4

21.6

30.8

1.85

0.34

5

27.0

35.8

1.70

0.91

6

23.1

34.6

1.79

0.12

7

20.9

37.8

1.85

0.03

8

24.5

33.7

1.85

0.34

9

22.0

30.3

1.63

1.72

10

24.5

30.2

1.79

3.57

11

19.7

32.2

1.70

0.13

12

24.0

30.7

1.99

1.00

13

23.2

34.6

1.79

0.13

  1. PBMC were activated with or without 10-6 M Dex, and Ct values for IL-9 were determined. The amplification efficiencies were measured for each sample, and were applied to the Ct differences between the Dex and no Dex samples to determine the proportion of IL-9 in samples treated with and without Dex.