Table 1 Primers and probes used for quantitative real-time RT-PCR

TLR1 (FP)

5'-CCCATTCCGCAGTACTCCATT-3'

TLR1 (RP)

5'-TTTCCTTGGGCCATTCCA-3'

TLR1 (TP)

5'-CAGTTATCACAAGCTCAAAAGTCTCATGGCCA-3'

TLR2 (FP)

5'-TGTGAAGAGTGAGTGGTGCAAGT-3'

TLR2 (RP)

5'-ATGGCAGCATCATTGTTCTCAT-3'

TLR2 (TP)

5'-TGAACTGGACTTCTCCCATTTCCGTCTTTT-3'

TLR3 (FP)

5'-CCTGGTTTGTTAATTGGATTAACGA-3'

TLR3 (RP)

5'-GAGGTGGAGTGTTGCAAAGGTAGT-3'

TLR3 (TP)

5'-CCCATACCAACATCCCTGAGCTGTCAA-3'

TLR4 (FP)

5'-AGCTCTGCCTTCACTACAGAGACTT-3'

TLR4 (RP)

5'-GCTTTTATGGAAACCTTCATGGA-3'

TLR4 (TP)

5'-CCCGGTGTGGCCATTGCTGC-3'

TLR5 (FP)

5'-GCACTTTTATCAATTGGCTTAATCAC-3'

TLR5 (RP)

5'-AACGAGTCAGGGTACACACAATATATG-3'

TLR5 (TP)

5'-CAATGTCACTATAGCTGGGCCTCCTGCAG-3'

TRAM (FP)

5'-CAGTGCTCTTACCCAGATGGA-3'

TRAM (RP)

5'-TCTGATAATCGATGACAGACTTCA-3'

TRAM (TP)

5'-CTGCCTGTGTTTCAATTCACGAAGCT-3'