Figure 8

mRNA expression of Toll-like Receptors (TLR) in stimulated primary small-airway-epithelial cells (SAEC). (A) SAEC were stimulated for 6 h (black bars) or 24 h (gray bars) with different type-1 cytokines. TLR1 – TLR6 mRNA expression was analyzed by quantitative RT-PCR. Results were normalized using GAPDH as endogenous control and are shown as fold changes relative to untreated controls. (B) SAEC were stimulated for 6 h (black bars) or 24 h (gray bars) with different type-2 cytokines. TLR1, TLR2 and TLR4 mRNA expression was analyzed by quantitative RT-PCR. Results were normalized using GAPDH as endogenous control and are shown as fold changes relative to untreated controls. No changes of the gene expression of TLR3, TLR5 and TLR6 were observed by stimulation of SAEC with type-2 cytokines (data not shown).