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Figure 6 | Journal of Inflammation

Figure 6

From: Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response

Figure 6

mRNA expression of genes involved in TLR signaling in stimulated primary small airway epithelial cells (SAEC). SAEC were stimulated for 6 h (black bars) or 24 h (gray bars) with different TLR ligands including LPS, flagellin, poly(I:C), macrophage activating lipopetide-2 (MALP-2) and zymosan. MyD88, TRIF, TOLLIP and IRAK-2 mRNA expression was analyzed by quantitative RT-PCR. Results were normalized using β-actin as endogenous control and are shown as fold changes relative to untreated controls. No changes of the gene expression of TRAM, TIRAP, IRAK-1, IRAK-3 and IRAK-4 were observed in the stimulated cells (data not shown).

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