Table 1 Sequences for Taqman RT-PCR. Sequences for primers and probes were designed using Primer Express (Applied Biosystems).
From: Nurr1 dependent regulation of pro-inflammatory mediators in immortalised synovial fibroblasts
Gene | Size of product | Primer | Sequence (5'-3') |
|---|---|---|---|
Nurr1 | 77 bp | Forward | TGTGTTCAGGCGCAGTATGG |
| Â | Â | Reverse | TCCCGAAGAGTGGTAACTGTAGC |
| Â | Â | Probe | CCTCGCCTCAAGGAGCCAGCC |
AREG | 70 bp | Forward | ACTCGGCTCAGGCCATTATG |
| Â | Â | Reverse | AAAATGGTTCACGCTTCCCA |
| Â | Â | Probe | TGCTGGATTGGACCTCAATGACACCTACT |
KITLG | 75 bp | Forward | TGGTGGCAAATCTTCCAAAAG |
| Â | Â | Reverse | CAATGACTTGGCAAAACATCCA |
| Â | Â | Probe | CATGATAACCCTCAAATATGTCCCCGGG |