Table 1 Sequences of the primers used in the RT-PCR reaction and the size of the amplified products of human and viral genes
Gene of interest | Size of the product | Primer orientation | Primer sequence |
|---|---|---|---|
β-actin | 307 bp | Forward Reverse | 5' AGCGGGAAATCGTGCGTG 3' 5' GGGTACATGGTGGTGCCG 3' |
hsf1 | 577 bp | Forward Reverse | 5' ATGGCCAGCTTCGTGCG 3' 5' ACAGCATCAGGGGCGTA 3' |
hsp70 | 590 bp | Forward Reverse | 5' TTTGACAACAGGCTGGTGAACC 3' 5' GTGAAGGATCTGCGTCTGCTTGG 3' |
hsp90 | 1498 bp | Forward Reverse | 5' GCTGTGCCGTTGGTCCTGTGC 3' 5' GGTTCTCCTTCATTCTGGTGC 3' |
E3L | 360 bp | Forward Reverse | 5' TATATTGACGAGAGTTCTGAC 3' 5' ACTCATTAATAATGGTGACAGG 3' |
F17R | 283 bp | Forward Reverse | 5' ATTCTCATTTTGCATCTGCTC 3' 5' AGCTACATTATCGCGATTAGC 3' |