Vaccinia virus-induced HSF1 redistribution. Cells uninfected (control) or infected for 24 h (MOI 5) were fixed and allowed to react with anti-human HSF1 antibodies (A) or stained with Hoechst 33258 (B). Panels A, B – epifluorescence, C – phase-contrast picture of the same cells. Representative images of three independent experiments are shown. The inserted bar – 20 μm.