RT-PCR analysis of the heat shock factor 1 and heat shock proteins in vaccinia virus infected human blood macrophages. HSF1, HSP70, HSP90α and β-actin mRNAs were measured at 3, 6, 16, 24, 48, 72 and 96 hours p.i. PCR data come from a single representative experiment being one of three separate experiments using the cells from healthy donors. Vaccinia virus (V) infection was carried out at MOI 1 (A) and 5 (B), control (C). HS – heat shock at 42°C for 1 h plus recovery at 37°C for 2 h. β-actin gene product was used as a control.