Functional characterisation of human pulmonary monocyte-like cells in lipopolysaccharide-mediated acute lung inflammation

Table 1 Primary antibodies used for flow cytometry and FACS

Fluorochrome-conjugated antibody	Clone	Manufacturer, catalogue number	Laser filter, bandpass
PMLC and Macrophages
Anti-human (h)CD14 PerCP/Cy5.5	HCD14	BioLegend, 325622	Blue, 695/40
Anti-hCD16 PE/Texas Red	3G8	Invitrogen, MHCD1617	Yellow/green (Y/G), 610/20
Anti-hHLA-DR V450	G46-6	BD Horizon, 561359	Violet, 450/50
Anti-hCD206 PE	15-2	BioLegend, 321105	Y/G, 582/15
Anti-hCD163 APC	GHI/61	BioLegend, 333609	Red, 670/30
Anti-hKi-67 PE	Ki-67	BioLegend, 350504	Y/G, 582/15
Anti-hCD71 FITC	CY1G4	BioLegend, 334104	Blue, 530/30
Anti-human mature macrophage marker (25 F9) APC	eBio25 F9	eBioscience, 14-0115-82	Red, 670/30
Regulatory T cells
Anti-hCD3 FITC	UCHT1	BD Pharmingen, 555332	Blue, 530/30
Anti-hCD4 APC/Cy7	RPA-T4	BioLegend, 300518	Red, 780/60
Anti-hCD25 PE	BC96	BioLegend, 302606	Y/G, 582/15
Anti-hCD127 PE/Cy5	eBioRDR5	eBioscience, 15-1278-42	Y/G, 670/30

Staining panels were prepared using specific combinations of antibodies in order to analyse multiple cell subpopulations within a single sample. FITC = fluorescein isothiocyanate; PE = phycoerythrin; APC = allophycocyanin.

ISSN: 1476-9255