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Figure 4 | Journal of Inflammation

Figure 4

From: Expression of pro-inflammatory mediators is inhibited by an avocado/soybean unsaponifiables and epigallocatechin gallate combination

Figure 4

Immunolocalization of NF-κB in chondrocytes. Immunofluorescence of NF-κB was analyzed by immunohistochemistry. (A) Left panel: Non-activated cells exhibited strong NF-κB cytoplasmic localization while nuclei remained unstained. Middle panel: Following cytokine activation, chondrocyte nuclei displayed intense fluorescence. Right panel: Chondrocytes pre-treated with ASU + EGCG did not show nuclear staining of NF-κB. Arrows point to nuclei. (B) Western blot: Relatively low levels of NF-κB nuclear protein were expressed in non-activated chondrocytes (Lane 1) with cytokine activation increasing NF-κB nuclear protein levels (Lane 2). Cells pre-treated with ASU + EGCG showed a decrease in nuclear NF-κB protein compared to activated controls (Lane 3).

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