Figure 8

Protease-resistant conformation in histatin 3-bound HSC70. HSC70 was mixed with control peptide (cont. pep.), P3a, or histatin 3, and the mixtures were analyzed by limited V8 protease proteolysis. The ratios of V8 protease to HSC70 were 2:1 and 20:1 (w/w). The mixtures were incubated at 30°C for 1.5 h, and the proteolytic fragments were separated on 12% SDS-PAGE gels and stained with Coomassie Brilliant Blue.