Figure 5

The effect of anti-TLR antibodies on HSC70-stimulated inflammatory cytokine production in HGFs. (A) IL-6 and (B) IL-8 production with LPS or HSC70 in the presence of anti-TLR2 and anti-TLR4 antibodies in HGFs. HGFs were cultured for 1 h after the addition of the antibodies and were then stimulated with LPSs or HSC70 for 24 h, and ELISAs for IL-6 and IL-8 were performed on the cultured media. (C) LPS- and HSC70-stimulated IL-6 and (D) IL-8 production in the presence of anti-CD14 antibody in HGFs. Assays were performed as described in the “Methods.” IgG served as the antibody control. Bars represent the means and range of duplicate samples. ***, P < 0.001 versus stimulation with LPS or HSC70 alone.