Table 1 Forward and reverse primers of TLRs 2–4, TLR7 and TLR9
Gene | Primer | Sequence |
|---|---|---|
TLR2 | Sense | 5′-GCCAAAGTCTTGATTGATTGG-3′ |
Antisense | 5′-TTGAAGTTCTCCAGCTCCTG-3′ | |
TLR3 | Sense | 5′-CGCCAACTTCACAAGGTA-3′ |
Antisense | 5′-GGAAGCCAAGCAAAGGAA-3′ | |
TLR4 | Sense | 5′-TGGATACGTTTCCTTATAAG-3′ |
Antisense | 5′-GAAATGGAGGCACCCCTTC-3′ | |
TLR7 | Sense | 5′-AGTGTCTAAAGAACCTGG-3′ |
Antisense | 5′-CCTGGCCTTACAGAAATG-3′ | |
TLR9 | Sense | 5′-GTGCCCCACTTCTCCATG-3′ |
Antisense | 5′-GGCACAGTCATGATGTTGTTG-3′ | |
HPRT1 | Sense | 5′-AGATGGTCAAGGTCGCAAGC-3′ |
Antisense | 3′-CATATCCTACAACAAACTTGTCTGGAA-5′ |