Decreased percentage of CD4+Foxp3+TGF-β+ and increased percentage of CD4+IL-17+ cells in bronchoalveolar lavage of asthmatics

Table 2 The sets of antibodies used in the study

Fluorochrome	Set Nr. 1	Set Nr. 2	Set Nr. 3
FITC	Anti- IFN-γ	Anti- IFN-γ	Anti- IFN-γ
PE	Anti- TGF-β	Anti- FoxP3	Anti- IL-8
PerCP-Cy5.5	Anti- TNF-α	Anti- MIP-1β	Anti- MIP-1β
PE-Cy7	Anti- IL-4	Anti- IL-4	Anti- IL-4
APC or Alexa Fluor 647	Anti- FoxP3	Anti- IL-10	Anti- IL-17A
APC-Cy7	Anti- CD25	Anti- CD25

FITC - Fluorescein isothiocyanate; PE - R-phycoerythrin; PerCP-Cy5.5 - a tandem fluorochrome composed of peridinin chlorophyll protein (PerCP) coupled to the cyanine dye Cy5.5; PE-Cy7 - a tandem fluorochrome composed of R-phycoerythrin (PE) coupled to the cyanine dye Cy7; APC - Allophycocyanin; APC-Cy7 - a tandem fluorochrome composed of Allophycocyanin (APC) coupled to the cyanine dye Cy7.

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