TLR inhibitors suppress IL-8 secretion from LPS, MALP-2 or Poly(I:C) treated IPE and RPE. IPE (A-D) and RPE (E-H) from the same donor were stimulated with LPS (serotype O111:B4; 10 μg/ml - maximal dose), MALP-2 (100 ng/ml) or Poly(I:C) (10 μg/ml) in the presence of OxPAPC (TLR2 and 4 inhibitors), CI-095 (TLR4 inhibitor) and chloroquine (TLR3 inhibitor) respectively, at various concentrations (□ = media only; ■ = LPS stimulation). Conditioned media were collected and assayed for IL-8 by ELISA. Data represents mean ± SD (N = 3). One-way ANOVA and Bonferroni’s multiple comparison test were used to analyse the data. IL-8 secretion by LPS, MALP-2 or Poly(I:C) treated IPE and RPE was significantly inhibited in the presence of OxPAPC, CI-095 or chloroquine in comparison to exposed to the ligand alone, *p < 0.05, **p < 0.01, ***p < 0.001. Results are representative of three experiments (two studies from the same donor and the results are consistent from three donors).