Dose response and time course of IL-8 secretion from LPS or Poly(I:C) treated IPE and RPE. Panel 1: Dose response; IPE (A and B) and RPE (C and D) from the same donor were stimulated with various concentrations of LPS (serotype O111:B4) or Poly(I:C) under serum free condition for 24 hours (control = basal media). Panel 2: Time course; LPS stimulation was performed at a maximal dose of 10 μg/ml under serum free condition for 24, 48 and 72 hours (□ = media only; ■ = LPS stimulation). Conditioned media were collected and assayed for IL-8 by ELISA. Data represents mean ± SD (N = 3). One-way ANOVA and Dunnett’s post test was used to compare LPS or Poly(I:C) treated samples to controls from panel 1, **p < 0.01, ***p < 0.001, ****p < 0.0001. Two-way ANOVA and Bonferroni’s multiple comparison test were used to analyse the data from panel 2. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Results are representative of three experiments.