Figure 1

Experimental protocol. Mice were randomly divided into six experimental groups (n = 6, each group) according to each target measurements: (1) Saline group, received an injection of saline (intraperitoneally: i.p.); (2) LPS group, received an injection of LPS (20 mg/kg, i.p.); (3) RIPC/Saline group, subjected to remote conditioning, followed immediately by an i.p. injections of saline; (4) Saline/RpostC group, received an injection of saline i.p., followed immediately by remote conditioning; (5) RIPC/LPS group, was subjected to remote conditioning, followed immediately by an injection of LPS (20 mg/kg, i.p.); (6) LPS/RpostC group, received an injection of LPS (20 mg/kg, i.p.), followed immediately by remote conditioning. Remote conditioning was induced with three 10-min ischemia/10-min reperfusion cycles of the right hind limbs using tourniquet.