Figure 3
From: Differential regulation of anti-inflammatory genes by p38 MAP kinase and MAP kinase kinase 6
Regulation of other p38-dependent anti-inflammatory genes. (a) DUSP1 mRNA expression was significantly reduced in p38 inhibitor-treated WT cells compared with WT control but not in MKK-deficient cells (*p < 0.05). TTP and IL-1RA mRNA levels were significantly reduced in the presence of the p38 inhibitor and MKK3−/− cells but not MKK6−/− cells compared with WT. (b) Western blot analysis showed reduced p38 phosphorylation in MKK3−/− BMDM but not MKK6−/− BMDM. TTP protein expression was similar in WT, MKK3−/− and MKK6−/−, although it was modestly elevated in MKK3−/− cells.