Time effect of Hcy and LPS on mRNA expression in M2 macrophage. (A) IL-6, (B) TNF-α, (C) iNOS, (D) IL-10, (E) Arg-1 and (F) MMR mRNA expression were assessed by real-time PCR. Gene expression is represented as fold-change compared to unstimulated macrophages. Data are representative of three independent experiments. (uns: unstimulated macrophages; Arg stands for Arg-1; *p < 0.05 vs uns; #p < 0.05 vs 6 h; Δp < 0.05 vs 12 h; &p < 0.05 vs 24 h).