Time effect of Hcy and LPS on mRNA expression of M1 markers genes. (A) IL-6, (B) TNF-α and (C) iNOS mRNA expression were assessed by real-time PCR. Gene expression is represented as fold-change compared to unstimulated macrophage. Data are representative of three independent experiments. (uns: unstimulated macrophages; *p < 0.05 vs uns, #p < 0.05 vs 6 h, Δp < 0.05 vs 12 h, &p < 0.05 vs 24 h).