Effects of Hcy and LPS on mRNA expression of M1 marker genes. (A) IL-6, (B) TNF-α and (C) iNOS mRNA expression at 12 h were assessed by real-time PCR. Gene expression is represented as fold-change compared to unstimulated macrophage. Data are representative of three independent experiments. (uns: unstimulated macrophages; *p < 0.05 vs uns, #p < 0.05 vs LPS, Δp < 0.05 vs LPS + Hcy 20 μmol/L, &p < 0.05 vs LPS + Hcy 50 μmol/L).