Figure 5
17-DMAG induces Hsp70 expression. Analysis of Hsp70 in PBMCs stimulated with 1 μg/ml plate-bound anti-CD3 antibody in absence and presence of different concentrations of 17-DMAG for 24 hours. Hsp70 protein expression was analyzed in cell lysates of these cell cultures by immunoblotting. Protein concentration was expressed relative to the β-actin level using densitometry measurements. The results are presented as mean values ± SEM of n = 3 healthy blood donors. *P < 0.05.