Figure 4
17-DMAG blunts NF κB p65 activity. Analysis of NFκB p65 in PBMCs stimulated with 1 μg/ml plate-bound anti-CD3 antibody in absence and presence of different concentrations of 17-DMAG for 24 hours. NFκB p65 activity and protein expression was analyzed in lysates of these cell cultures by ELISA and immunoblotting, respectively. Protein concentration was expressed relative to the β-actin level using densitometry measurements. The results are presented as mean values ± SEM of n = 3 healthy blood donors. *P < 0.05.