Figure 2
17-DMAG reduces frequencies of proinflammatory Th1 and Th17 cell subpopulations. PBMCs were stimulated with 1 μg/ml of plate-bound anti-CD3 antibody in absence or presence of 17-DMAG (0.1 μM) for 72 hours. GolgiStop™, PMA, and ionomycin were added in the last 5 hours of culture before permeabilization. Cells were washed, stained with anti-CD4, anti-IFN-γ, and anti-IL-17 antibodies, and analyzed by flow cytometry. The numbers in upper right quadrants of the representative dot blots show the percentage of positive cells of each CD4+ T cell subpopulation. The results are presented as mean values ± SEM of n = 4 healthy blood donors. *P < 0.05.