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Figure 1 | Journal of Inflammation

Figure 1

From: Inhibitory effects of heat shock protein 90 blockade on proinflammatory human Th1 and Th17 cell subpopulations

Figure 1

17-DMAG inhibits proliferation of T cells. A Proliferation response of human PBMCs stimulated by plate-bound anti-CD3 antibody (1 μg/ml) in absence and presence of 17-DMAG. Cell cultures were exposed to different concentrations of 17-DMAG and proliferation was assayed by ELISA after BrdU incorporation on the 6th day of treatment followed by 24 hours incubation. B LDH-based cytotoxicity ELISA measurement in culture medium after 24-hour incubation of PBMCs with different concentrations of 17-DMAG. LDH release from cells lysed with 1% Triton X-100 was regarded as 100%. The results are presented as mean values ± SEM of n = 4 healthy blood donors. *P < 0.05.

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