Figure 1

Inhibition of Rb⁺incorporation into lung tissue by OA and ouabain. (A) Control group was treated solely with 8 μmol Rb⁺. In the experimental groups, 2.5, 5 and 10 μmol of OA; 10^-3 or 10^-4 μmol of ouabain; or 10^-4 μmol ouabain plus OA 5 μmol were injected by the i.v. route together with 8 μmol Rb⁺. Rb⁺ incorporation in lungs was measured after 30 min by ICP-OES in mineralized lung tissues. The results are expressed in μmol Rb⁺ incorporated per h per g of wet tissue ± SEM of 6 to 15 animals in each group. (B) Calculated percent inhibition of Na/K-ATPase based on data from Figure1A (the difference between Rb⁺ incorporation in absence and in presence of ouabain was considered as 100% enzyme activity). *P < 0.0001, compared to controls.