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Figure 5 | Journal of Inflammation

Figure 5

From: TLR2 stimulation induces cardiac inflammation but not cardiac depression in vivo

Figure 5

Sacomere shortening of isolated cardiomyocytes after incubation with LTA (10 μg/ml). A-D: Averaged original recordings of sarcomere shortening from WT- and TLR2-D cardiomyocytes with LTA or in culture medium (CM) alone. After 30 s stimulation-pause cells were stimulated at 1 Hz. The mean peak values (± SEM) of sarcomere shortenings were fitted by a double exponential function to give an impression of the time course of the staircase. Lipoteichoic acid treatment reduced shortening amplitudes only in WT-cells (plots averages of n = 5). E-G: Shortening-frequency plots of steady-state sarcomere shortening. Sarcomere shortening of WT- and TLR2-D cardiomyocytes was recorded within 60 min after isolation (Tyrode) or 6 h after incubation with LTA or in CM alone (E, F; * LTA vs. Tyrode, # LTA vs. CM). After 5 h of incubation with LTA in CM one group was transferred to CM with LTA supplemented with the iNOS-inhibitor SMT (LTA + SMT) (G; * LTA vs.CM, # LTA vs. LTA + SMT; E-G: n = 10–38 cells; mean ± SEM).

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