Figure 2

The viability of HGFs after miRNA-146 inhibition. (A) HGFs were transfected with 10 and 100 nM miRNA-146a and miRNA-146b-5p and stimulated with 1 μg/ml of P.g LPS for 24 hours. Total RNA from the HGFs was harvested at different times for the quantitative RT-PCR assays. (B) The HGFs were collected and separated by FACS based on PI and Annexin V labeling. The numbers indicate the ratios of necrosis and apoptosis. The results shown represent one of three independent experiments.