Table 1 Primers used for amplification of SP-A1, SP-A2 and SP-A
Target Genes | Primer Sequences | Tm(°C) | Cycles | Length(bp) |
|---|---|---|---|---|
SP-A | F-GAAGGACGTTTGTGTTGGAA | 56.1 | 35 | 439 |
commom to SP-A1 and A2 | R-TGGATTCCTTGGGACAGCAA | |||
SP-A | F-CACTTTTGATGCCATTCAGGAG | 56.3 | 35 | 295 |
commom to SP-A1 and A2 | R- GGTCAGTCGGGAGTACAGGC | |||
SP-A1 | F-TGGGCAAGGTAATCAGTG | 49.1 | 33 | 365 |
R-GTCCAGGAAGATGGGTTT | ||||
SP-A2 | F-TATTGACCGAGCACATACC | 49.3 | 33 | 212 |
R-GTCCAGGAAGATGGGTTT | ||||
GADPH | F-TCCCTGGAGAAGAGCTACGA | 53.5 | 30 | 200 |
R-TCGTCATACTCCTGCTTGCT |