Transcription networks responsible for early regulation of Salmonella-induced inflammation in the jejunum of pigs

Table 2 Percent inhibition or stimulation of Salmonella -induced IL8 and NFKBIA mRNA expression

Chemical	Key factor genes (3 listed)	CAS # chemical group		NFKBIA		IL8
			μM	%	%	%	%
			hours	4	20	4	20
Genistein	FOS	446-72-0	0.5	80	57	18	54
	CEBPB & D	Isoflavones	5	71	56	12	66
	PITX2		50	53	27	3	11
Quercetin	IL1B	117-39-5	2	92	61	86	90
	NFKBIA	Flavonols	20	111	75	86	92
	F3		200	241	52	19	26
Indomethacin	EGR1	53-86-1	1	106	66	86	93
	NR4A1	Indoles	10	123	129	86	101
	NFKBIA		100	261	108	19	42
			hours	3	6	3	6
Curcumin	ETS2	458-37-7	1	186	111	275	138
	CEBPB	Diarylheptanoids	10	412	100	843	37
	NFE2L2		100	27	10	86	24
Chenodeoxycholic Acid	PPARG	474-25-9	1	217	145	291	317
	NR4A1	Bile Acids and Salts	10	407	140	468	293
	XBP1		100	591	211	779	318
Nordihydroguaiaretic Acid	HSD11B2	500-38-9	1	226	118	141	85
	FOS	Lignans	10	222	78	214	123
	IL8		100	15	6	2	1
(E)-Guggulsterone	NFKBIA	6439929	1	135	45	77	308
	NFE2L2	Plant Gums	5	96	32	69	139
	STAT3		50	21	8	21	13
(Z)-Guggulsterone	NFKBIA	6450278	1	75	43	23	176
	NFE2L2	Plant Gums	5	46	35	18	122
	STAT3		50	47	16	10	4
Sodium Selenite	JUNB	10102-18-8	10	83	31	96	44
Sodium Selenite	FOS	Selenium Compounds	100	100	37	115	37
	PITX2		1000	143	53	30	12

IL8 and NFKBIA mRNA expression was measured with QRT-PCR in IPEC-J2 cells in the absence or presence of different concentration of chemicals (μM), after 4 and 20 hours (top panel) or after 3 and 6 hours (bottom panel) of incubation. Chemicals were selected based on their association with the in this study identified key transcription factor/regulators genes (maximal 3 genes were listed per chemical). For each chemical CAS numbers (#) and the group of chemicals to which they belong were listed.

ISSN: 1476-9255