Figure 3

Identification of PBMC subsets producing IL-10 in response to IF7 mAb. PBMC from 10 healthy donors were surface stained with anti-CD36 or anti-CD14, fixed, permeabilized and incubated with anti-human IL-10 or matched isotype control. CD36⁺ lymphocytes and CD14⁺ monocytes were analyzed for intracellular IL-10 as shown (a). Bar graphs show mean values ± SE (b-c). Significant differences between stimulation with mAb 1F7 and the IgMκ control mAb control are indicated (*p < 0.05). For 5 individuals, CD14⁺ monocytes were depleted and the remaining cells incubated with 1.92 μg/ml 1F7 mAb or IgMκ control mAb for 24 h, after which IL-10 in culture supernatants was measured (d). The mean IL-10 concentration in supernatant was significantly different between 1F7 mAb-treated, monocyte-depleted PBMC and intact PBMC (*p < 0.05) and between 1F7 mAb and IgMκ control mAb-treated monocyte-depleted PBMC (#p < 0.02).