The downstream effect of PKC β inhibition on oxidative burst. Cells were pretreated with LY333531 (Ly) at increasing concentrations and NADPH oxidase activity measured by DHR fluorescence on flow-cytometry. DPI and Ro-31-8220 (Ro) were used as positive controls. LY333531 reduced NADPH oxidase activity at both 100 nM (p<0.01) and 1 μM (p<0.001) consistent with the concentrations of inhibitor required to reduce NET formation. # indicates p<0.05, ## indicates p<0.01, and ### indicates p<0.001 vs. untreated control. ** indicates p<0.01 and *** indicates p<0.001 vs. PMA treated cells.