Figure 3

Effect of isozyme specific PKC inhibitors on NET formation: Cells were plated as described and either pre-treated with at increasing concentrations of Gö 6976 (conventional PKC inhibitor), Rottlerin (atypical PKC inhibitor), PKCζ psuedosubstrate (novel PKC inhibitor), or control media, before stimulation with PMA at 10 nM for 4 h. A) Pre-treatment with Gö 6976 significantly reduced NET formation at 100 nM (P<0.05) and 1 μM (p<0.001). B) Pre-treatment with Rottlerin had no effect on NET formation. C) Pre-treatment with PKCζ psuedosubstrate had no effect on NET formation. Data show mean +/− SEM for n=3 independent experiments. * indicates p<0.05, *** indicates p<0.001.