Annexin-A1 peptide down-regulates the leukocyte recruitment and up-regulates interleukin-10 release into lung after intestinal ischemia-reperfusion in mice

Table 1 AnxA1 Immunohistochemistry analysis in lung leukocytes after intestinal ischemia-reperfusion

	Control	Intestinal I/R		Intestinal I/R + Ac2-26
	Control	2 h	24 h	2 h	24 h
Intravascular PBMN	49,7 ± 7,2	55.9 ± 4.5	83,7 ± 3,1***	65.4 ± 5.3	60,5 ± 5,2#
Alveolar monocyte/macrophage	43,2 ± 5,5	43.1 ± 4.4	66,7 ± 3,7*	44.4 ± 2.7	46,3 ± 7,3
Connective tissue monocyte/macrophage	60,2 ± 1,7	53.8 ± 2.1*	64,9 ± 1,9	45.6 ± 1.8***#	55,8 ±1,9#
Intravascular neutrophils	88,5 ± 4,2	71.3 ± 2.2***	83,6 ± 2,7	72.6 ± 2.5***	65,9 ± 3,0***###
Alveolar neutrophils	----	59.0 ± 3.8	80,6 ± 3,7	47.4 ± 3.4	68,1 ± 3,5
Connective tissue neutrophils	80,9 ± 2,6	71.2 ± 2.3	95,8 ± 2,9***	69.3 ± 2.4*	70,3 ± 1,8*###

AnxA1 protein expression was analyzed in mice lung at 0, 2 and 24 h post-intestinal ischemia-reperfusion (n = 10 per group). Groups of mice were treated with the peptide Ac2-26 (i.p.) as described in Material and Methods section. Values (densitometric units) are mean ± SEM of 10 tissue sections analyzed from five mice per group. *P < 0.05, **P < 0.01, ***P < 0.001 versus control group values. #P < 0.05, ##P < 0.01, ###P < 0.001 versus corresponding intestinal I/R group values.

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