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Figure 4 | Journal of Inflammation

Figure 4

From: Annexin-A1 peptide down-regulates the leukocyte recruitment and up-regulates interleukin-10 release into lung after intestinal ischemia-reperfusion in mice

Figure 4

Endogenous annexin-A1 expression. (A) Leukocytes from control group display basal immunostain for AnxA1 protein (arrow). (B) After intestinal I/R, expression of the protein was augmented in the cytosol of lung connective tissue leukocytes (arrows). (C) Peptide Ac2-26 pretreatment decreases expression of AnxA1 in the leukocytes (arrows). Vessel (V). Haematoxylin-eosin counterstained. Bar, 10 μm. (D) AnxA1 protein content was also analyzed by Western blotting. A representative image of five different experiments indicates that AnxA1 expression (37 kDa; whole protein) in the lung tissue was down regulated after peptide Ac2-26 treatment. The cleaved AnxA1 (33 kDa) was observed in the control groups, increased after 2 h of intestinal ischemia-reperfusion and weakly present after peptide Ac2-26 treatment. Data were analyzed by densitometry as described in material and methods.

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