Zinc supplementation alters airway inflammation and airway hyperresponsiveness to a common allergen
© Morgan et al; licensee BioMed Central Ltd. 2011
Received: 30 August 2011
Accepted: 7 December 2011
Published: 7 December 2011
Zinc supplementation can modulate immunity through inhibition of NF-κB, a transcription factor that controls many immune response genes. Thus, we sought to examine the mechanism by which zinc supplementation tempers the response to a common allergen and determine its effect on allergic airway inflammation.
Mice were injected with zinc gluconate prior to German cockroach (GC) feces (frass) exposure and airway inflammation was assessed. Primary bone marrow-derived neutrophils and DMSO-differentiated HL-60 cells were used to assess the role of zinc gluconate on tumor necrosis factor (TNF)α expression. NF-κB:DNA binding and IKK activity were assessed by EMSA and in vitro kinase assay. Protein levels of A20, RIP1 and TRAF6 were assessed by Western blot analysis. Establishment of allergic airway inflammation with GC frass was followed by administration of zinc gluconate. Airway hyperresponsiveness, serum IgE levels, eosinophilia and Th2 cytokine production were assessed.
Administration of zinc gluconate prior to allergen exposure resulted in significantly decreased neutrophil infiltration and TNFα cytokine release into the airways. This correlated with decreased NF-κB activity in the whole lung. Treatment with zinc gluconate significantly decreased GC frass-mediated TNFα production from bone-marrow derived neutrophils and HL-60 cells. We confirmed zinc-mediated decreases in NF-κB:DNA binding and IKK activity in HL-60 cells. A20, a natural inhibitor of NF-κB and a zinc-fingered protein, is a potential target of zinc. Zinc treatment did not alter A20 levels in the short term, but resulted in the degradation of RIP1, an important upstream activator of IKK. TRAF6 protein levels were unaffected. To determine the application for zinc as a therapeutic for asthma, we administered zinc following the establishment of allergic airway inflammation in a murine model. Zinc supplementation decreased airway hyperresponsiveness and serum IgE levels, but had no effect on Th2 cytokine expression.
This report suggests that the mechanism by which zinc supplementation alters NF-κB activity is via the alteration of A20 activity. In addition, this study provides evidence that supplementation of zinc to asthmatics may alter airway reactivity and serum IgE levels, suggesting zinc supplementation as a potential treatment for asthmatics.
Zinc is an essential trace element acquired by dietary means. It plays a central role in modulating the immune system and is essential for cellular function in the immune response as well as acting as an antioxidant . The syndrome of zinc deficiency in humans is characterized by susceptibility to infections and in a mouse model of polymicrobial sepsis, zinc deficiency increased organ damage and mortality . Zinc is known to modulate the immune system via the NF-κB pathway, a ubiquitous transcription factor that controls many immune response genes including cytoplasmic cytokines, decreasing the inflammatory response; however the mechanism by which this occurs is currently unclear . A20 is an important regulator protein of NF-κB, acting as an inhibitor to shut down its activation. Its transcription is induced by NF-κB, suggesting a negative feedback loop to temper inflammation . A20 is also a seven zinc finger protein that functions as an ubiquitin-editing enzyme downstream in the TNFR (tumor necrosis factor receptor 1) and TLR (toll-like receptor) pathways toward NF-κB activation. In order to inhibit NF-κB activation, A20 alters the ubiquitination of receptor-interacting protein 1(RIP1), a protein crucial for the activation of IKK, to target it for degradation and also inactivates TNF receptor-associated factor 6 (TRAF6) by inhibiting its polyubiquitination . Thus A20, as a critical player in suppressing NF-κB activation  and as a zinc-containing protein, became a potential target for the location of zinc's effect in immune modulation.
Because of its immune modulating effects, zinc has also gained considerable interest with respect to airway inflammation and asthma. Many articles have documented a relative zinc deficiency in asthmatics, or patients who wheeze, implicating a loss of inflammatory modulation as a potential initiator of symptoms. Serum zinc levels in asthmatics have been found to be lower when compared to non-asthmatics [7, 8]; hair zinc levels were lower in wheezy infants when compared to healthy controls ; and finally, zinc levels in the sputum of asthmatics were significantly lower than in healthy subjects and were associated with increased frequency of wheeze, severity of asthma, and worse lung function . With this data in mind, and with the ability to supplement zinc in an already established model of airway inflammation, we also sought to examine zinc's effect on airway inflammation and reactivity with the hope that it may be considered as a therapeutic medication in relevant patients.
In these studies, we were able to reaffirm zinc's action as an anti-inflammatory agent via inhibition of the NF-κB pathway and present evidence that zinc alters the activity of inhibitory protein A20. In addition, our data support a role for zinc supplementation in decreasing airway reactivity and serum IgE levels in a murine model of experimental allergic airway inflammation.
Materials and methods
Fecal remnants (frass) were collected from German cockroaches (Blattella germanica) and reconstituted as previously described in endotoxin-free PBS . The frass preparation was frozen in aliquots and the same batch was used throughout the experiments.
Animals and GC frass exposure
Assessment of airway inflammation
Lungs were lavaged thoroughly with 1 ml of Hanks balanced salt solution (HBSS) without calcium or magnesium. The bronchoalveolar lavage fluid (BAL) was centrifuged (1,800 rpm for 10 min); the supernatant was removed for TNFα analysis by ELISA (R&D Systems, Minneapolis, MN) and immediately stored at -80°C. Total cell numbers were counted on a hemocytometer. Smears of BAL cells prepared with a Cytospin II (Shandon Thermo, Waltham, MA) were stained with Diff-Quick (Thermo Electron Corporation, Pittsburg, PA) solution for differential cell counting.
Primary mouse bone marrow-derive neutrophils
Bone marrow from femurs and tibias was isolated, rinsed and red blood cells were lysed. Resuspended cells were layered onto a three step Percoll gradient (52%, 64%, 72%) and centrifuged (1,000 rpm for 30 min at RT). The bottom layer (64%-72%) containing neutrophils was collected, counted and plated as previously described . Cells (1 × 106) were cultured in MEM medium supplemented with 10% fetal bovine serum 50 μg/ml streptomycin, 2 U/mL penicillin, and 2 mM L-glutamine for treatment.
HL-60 promyelocytic leukemia cells (ATCC, Manassas, VA) were cultured in MEM medium and were differentiated in the presence of 1% DMSO for 3 days. Cells were centrifuged, washed and deprived of serum for 6 h. For all cell culture experiments, cells were treated with the following concentrations of GC frass (1 μg/ml), LPS (1 μg/ml; from E. Coli 055:B5, Sigma Chemical Corp, St. Louis, MO) in the presence or absence of zinc gluconate (1 μM; Spectrum Chemicals, New Brunswick, NJ) and the zinc ionophore, pyrithione (10 μM; 2-mercapopyridine N-oxide, Sigma Chemical Corp). In all cases, zinc was added in the presence of pyrithione, except where noted. Lactase dehydrogenase (LDH) cytotoxicity assay kit (US Biological, Marblehead MA) was performed on cell supernatants to determine if zinc gluconate and/or pyrithione treatment resulted in cell death.
Assessment of TNFα production
Following treatment, cell culture media was clarified by centrifugation (10,000 g × 10 min) and immediately stored at -80°C. The fluid was analyzed for TNFα using an ELISA kit from R&D Systems (Minneapolis, MN).
Electrophoretic mobility shift assay (EMSA)
HL-60 cells were treated as described for 1 h. Cells were harvested and nuclear proteins isolated as previously described . All nuclear extraction procedures were performed on ice with ice-cold reagents. Protein concentrations were determined by Bradford assay (Bio-Rad, Hercules, CA) and stored at -70°C until use. The probe was labeled with [γ-32P]ATP using T4 polynucleotide kinase (Invitrogen, Carlsbad, CA) and purified in MicroBiospin chromatography column. The gel was run using 10 μg of nuclear protein as previously described . An oligonucleotide probe encoding the consensus sequence of NF-κB was purchased from Santa Cruz (Santa Cruz, CA). Non-radioactive specific and non-specific probes were added at 5× the concentration of the radiolabeled probe. Gels were transferred to Whatman 3 M paper, dried under a vacuum at 80°C for 1 h, and exposed using a Phosphoimager.
HL-60 cells were treated as described above for 1 h. Cellular lysate was harvested, and IKK was immunoprecipitated using an IKKγ antibody bound to protein G beads. The beads were pulled down and incubated with GST-IκBα and 32P-ATP. The samples were isolated by gel electrophoresis and exposed to film. An IKKγ (Santa Cruz, Santa Cruz, CA) Western blot was performed to confirm equal immunoprecipitation.
Western blot analysis
Following treatment, whole cell lysates were isolated and separated on an 8-16% Tris-glycine gel (Invitrogen, Carlsbad, CA). A20 (eBioscience, San Diego, CA), RIP1 (R&D Systems, Minneapolis, MN), TRAF6 and β-actin (both from Santa Cruz Biotechnology, Inc., Santa Cruz, CA) were detected by Western Blot analysis.
Murine model of allergic airway inflammation
For airway administration of allergen, mice were given three intratracheal inhalation challenges of PBS (40 μl) or GC frass (40 μg/40 μl) on days 0, 7, and 14 (n = 6 mice/group). On days 14, 15, and 16, mice were injected i.p. with either PBS or zinc gluconate (10 mg/kg). Mice were harvested on day 17 for allergic airway responses (Figure 1B).
Airway hyperresponsiveness measurements
Allergen-induced airway hyperresponsiveness (AHR) was determined as we have previously described . Briefly, mice were anesthetized 72 hours after the last GC frass exposure, intubated and ventilated at a rate of 120 breaths per minute with a constant tidal volume of air (0.2 ml), and paralyzed with decamethonium bromide (25 mg/kg). After establishment of a stable airway pressure, 25 μg/kg weight of acetylcholine was injected i.v. and dynamic airway pressure (airway pressure time index [APTI] in cm-H2O × sec-1) was followed for 5 minutes.
Animals were bled and serum isolated for total IgE levels. Serum was also analyzed for zinc levels using the QuantiChrom Zinc Assay kit (BioAssay Systems, Hayward, CA).
Liberase/DNase I digests of the lung were prepared to obtain single lung cell suspensions. Single cell suspensions (2.5 × 105) were incubated for 72 hours in culture medium (RPMI) or in RPMI treated with Conconavalin A (10 μg/ml) and supernatants were analyzed by ELISA for cytokine expression as previously described .
When applicable, statistical significance was assessed by one-way analysis of variance (ANOVA). Differences identified by ANOVA were pinpointed by Student-Newman-Keuls' multiple range test.
Zinc supplementation reduced GC frass-induced airway inflammation
Zinc directly affects neutrophil cytokine production via NF-κB
To explore the mechanism by which zinc gluconate regulated TNFα production, we asked whether zinc gluconate could inhibit NF-κB activation and DNA binding. HL-60 cells were differentiated in DMSO for 3 days prior to treatment with GC frass or LPS in the absence or presence of zinc gluconate and pyrithione. As expected, both GC frass and LPS increased NF-κB-DNA binding (Figure 4C). Importantly, NF-κB-DNA binding was completely attenuated with zinc supplementation. Next, we performed a time course of GC frass and LPS treatment in the absence and presence of zinc supplementation and determined the levels of IκBα. We found increased degradation of IκBα following treatment with both GC frass and LPS (data not shown). Zinc supplementation resulted in the sustained presence of IκBα, suggesting less degradation of the protein. Since IκBα phosphorylation and thus degradation is mediated by IKK activation, we asked whether zinc gluconate regulated IKK activity as determined by an in vitro kinase assay. GC frass-induced activation of IKK was attenuated in the presence of zinc gluconate (Figure 4D). Together these data suggest a role for zinc gluconate inhibition of NF-κB upstream of IKK activation.
Regulation of A20 by zinc gluconate
Zinc alters the activity of A20
Zinc supplementation decreased allergic airway inflammation in mice
In this report, we describe a role for zinc supplementation in modulating the innate immune response to a common allergen in vivo which is characterized by cellular infiltration and cytokine release into the airways of mice. In addition, we found that zinc supplementation is sufficient to decrease airway hyperresponsiveness and serum IgE levels suggesting an important therapeutic treatment. Our data suggests that zinc works to temper inflammation at the level of A20 within the NF-κB pathway. A20 is a zinc-fingered protein that is a well-known inhibitor of the NF-κB pathway and was thus a good potential target for the location of zinc's anti-inflammatory effect within this pathway. In our study, we found that zinc supplementation, when added concurrently with GC frass, did not affect A20 levels in the short term (4 h), but resulted in decreased A20 protein levels at 18 h. We postulate that this was most likely due to transcriptional regulation with the enhancement of the NF-κB negative feedback loop and inhibition of continued activation of NF-κB, thus eventually decreasing A20 protein production. It has been shown that zinc can induce increased A20 levels in the presence of a variety of stimuli including LPS  and phorbol myristate acetate (PMA) . These experiments differed from ours in that media containing zinc was added to cells for 10 days prior to stimulation, but they support our interpretation that zinc regulates A20 to alter NF-κB activity.
Our results indicate a potential mechanism by which zinc regulates NF-κB activity; i.e. by enhancing the activity of A20 thus resulting in de-ubiquitination of RIP1 and leading to its degradation. The ubiquitin editing abilities of A20 were recently examined and better defined. Shembade et. al.  showed that A20 removes the activating chains of ubiquitin from locus K63 on RIP1 and facilitates the addition of inhibitory chains to locus K48, targeting RIP1 for degradation and thus preventing the activation of IKK downstream. They also showed that A20 inhibits the polyubiquitinization and activation of TRAF6, a process that is dependent on Ubc13 enzymatic activity. Ubc13 is also ubiquitinated at K48 by A20 and targeted for degradation, but this occurs late after stimulation. TRAF6 is then rendered inactive, though not degraded . Utilizing these findings, we were able to evaluate A20 activity using RIP1 and TRAF6 protein levels as a surrogate. With respect to A20 activity, we were able to show that RIP1 was degraded more quickly in the presence of zinc gluconate after stimulation with GC frass, but TRAF6 levels remained unchanged. These findings suggest that the addition of zinc gluconate augments the activity of A20; enabling it to act more rapidly on RIP1 resulting in its degradation and thus ceasing the NF-κB inflammatory cascade more quickly. A limitation of the current study was our evaluation of A20 activity via RIP1 as an indirect assessment; however our studies clearly show that in the presence of zinc, RIP1 degradation is enhanced, suggesting an increased proteosomal targeting due to altered ubiquitin states. Our findings suggest that zinc is acting on the NF-κB pathway at the level of A20 to further enhance its inhibitory effects.
In this study we also wanted to investigate zinc as an anti-inflammatory with potential therapeutic utility for airway inflammation. Asthma is a chronic inflammatory disease of the airways and is characterized by increased mucus production and airway hyperresponsiveness. Several studies have shown a role for increased NF-κB in asthmatics, including increased NF-κB p65 protein abundance, IκBα phosphorylation and IKK activity in peripheral blood mononuclear cells (PBMC) of uncontrolled asthmatics compared to normal individuals  and greater levels of NF-κB p65 and p50 activation in cultured bronchial epithelial cells from untreated asthmatics than controls . NF-κB was also found to be upregulated in bronchial epithelial cells a murine model of ovalbumin-induced allergic airway inflammation . The importance of NF-κB signaling in allergic inflammation and mucus production was shown using mice with IKK-deficient Clara epithelial cells . In this study, we found a significant reduction in airway hyperresponsiveness in our murine model by administering zinc gluconate for three days following the final allergen exposure. Importantly, the levels of serum IgE were substantially decreased following zinc administration. IgE is known to bind to specific Fcε receptors on mast cells thus result in release of histamine and other inflammatory substances to produce an allergic cascade. Zinc supplementation did not appear to have any effect on levels of the Th2 cytokines IL-13, IL-5 and IL-4. Much of the literature on zinc and asthma has focused primarily on levels of dietary zinc; however Lang et. al.  recently showed that zinc supplementation following establishment of allergic inflammation reduced the levels of eosinophils and lymphocytes in the BAL fluid of mice. They failed to find a significant difference in the amount of mucus hyperplasia, determined by PAS staining, following zinc administration however. We also found decreased numbers of eosinophils and neutrophils in the BAL fluid of mice; however unlike neutrophil levels, the decrease in eosinophil levels did not reach statistical significance. It is possible that the amount of times zinc is administered (i.e. 3 times in our protocol compared to twice a week from days 34-52 in the Lang protocol) was responsible for the different results. To our knowledge, this is the first report showing that zinc supplementation can modulate airway responsiveness to cholinergic agents.
The literature on zinc as a potent antioxidant is vast but what role this may have in airway inflammation remains unclear. Recent studies have looked at abnormal distributions of trace minerals, including zinc, as an instigator of oxidative damage and inflammation in asthma [25, 26]. Prasad et. al.  investigated the role of zinc as an antioxidant and after supplementation to human subjects, noted a decrease in oxidative stress and NF-κB activation in isolated mononuclear cells when compared to placebo. TNFα and IL-1β are inflammatory cytokines known to exert oxidative stress on cells via the generation of reactive oxygen species (ROS). In their study, they also noted a decrease in TNFα and IL-1β cytokine levels in their volunteers who took zinc supplements, suggesting that one way in which zinc functions as an antioxidant is by negatively regulating the gene expression of these inflammatory cytokines to prevent the formation of ROS . Thus, zinc's role as an antioxidant likely works concurrently with its anti-inflammatory role via similar or overlapping biochemical mechanisms. Similar to Prasad et. al., we were able to show that zinc negatively inhibits NF-κB-induced cytokine production. We acknowledge that some of the positive effects noted on airway hyperresponsivity and inflammation may have been an antioxidant effect of zinc supplementation. However, we continue to promote the supplementation of zinc as a therapeutic to temper the inflammation as well as the oxidative stress induced by allergic stimuli in the airways.
Zinc gluconate is an inexpensive medication, readily available and administered both parenterally and orally. With the severity and prevalence of asthma on the rise, our data suggest the possibility of a readily available, easily dosed, and cost effective manner to aid in the suppression of airway hyperresponsiveness.
List of abbreviations
I kappa B alpha
I kappa kinase
nuclear factor kappa B
receptor-interacting protein 1
reactive oxygen species
tumor necrosis factor alpha
tumor necrosis factor receptor
TNF receptor-associated factor 6.
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